![]() CR Biol 331:806–814īarreto LC, Garcia QS (2017) Accelerated ageing and subsequent imbibition affect seed viability and the efficiency of antioxidant system in macaw palm seeds. Physiol Plantarum 97:104–110īailly C, El-Maarouf-Bouteau H, Corbineau F (2008) From intercellular signalling networks to cell death: the dual role of reactive oxygen species in seed physiology. Seed Sci Res 14:93–107īailly C, Benamar A, Corbineau F, Côme D (1996) Changes in malondialdehyde content and superoxide dismutase, catalase and glutathione reductase activities in sunflower seeds as related to deterioration during accelerated aging. Elsevier, Amsterdam, pp 227–287īailly C (2004) Active oxygen species and antioxidants in seed biology. In: Kyle DJ, Osmond CB, Arntzen CJ (eds) Photoinhibition. Methods Enzymol 105:121–126Īsada K, Takahashi M (1987) Production and scavenging of active oxygen in photosynthesis. glyptostroboides seeds have a low level of vigor and poor ability to maintain vigor, which is probably associated with the inefficiency of its enzymatic antioxidative system.Īebi H (1984) Oxygen radicals in biological systems. We used germination tests, relative electrical conductivity and malondialdehyde content determination, ultrastructural observation of embryo cells, and analysis of superoxide dismutase, catalase, ascorbate peroxidase, and dehydroascorbate reductase activities during accelerated aging treatment. Our objective was to elucidate causes of the low germination rate in an effort to enhance potential for conservation of the species. The present study aimed to describe the aging mechanism of M. Consequently, population regeneration by seed is low under natural conditions, which probably contributes to the endangered status of this plant species. However, its seeds germinate at a very low percentage. PacBio-Sequence RBOH ROS WGCNA germination percentage oxidative phosphorylation.Metasequoia glyptostroboides is an endangered relict plant native to China that has been widely introduced into many countries and areas around the world. These findings provide a broader perspective for studying the regulatory mechanism of seed aging and a large number of potential target genes for the breeding of other endangered gymnosperms. ![]() Weighted gene co-expression network analysis (WGCNA) revealed that the dark magenta, orange, and medium purple modules were highly correlated with physiological indicators such as SOD, CAT, and GSH and further identified 40 hub genes such as Rboh, ACO, HSF, and CML as playing important roles in the antioxidant network of M. A total of 15,376 differentially expressed genes (DEGs) were obtained KEGG enrichment analysis results revealed that seed aging may be mainly involved in the protein-processing pathways in endoplasmic reticulum, oxidative phosphorylation, and ascorbate and aldarate metabolism. A total of 42,189 unigenes were identified in the whole transcriptome, and 40,446 (95.86%) unigenes were annotated in at least one protein database. ![]() -) content and production rate significantly decreased, while malondialdehyde (MDA) and hydrogen peroxide (H 2O 2) content and glutathione peroxidase (GPX) and catalase (CAT) activity gradually increased during the aging process.Superoxide dismutase (SOD) activity, ascorbic acid (AsA), glutathione (GSH) content and superoxide anion (O 2 glyptostroboides seeds was 54 ± 5.29%, and significantly declined to 9.33 ± 1.88% after 6 days of aging, and then gradually decreased until the seed died on day 8. It was found that the germination percentage of fresh dried M. glyptostroboides seeds, we analyzed the antioxidant system and transcriptome at 0, 2, 4, 6, and 8 days after artificial accelerated aging treatment at 40 ☌ and 100% relative humidity. In order to investigate the physiological and molecular mechanisms underlying the aging process of M. glyptostroboides seeds, and the physiological mechanism of seed aging in M. There is a risk of extinction due to the low vigor of M. Cheng, as the only surviving relict species of the Taxodiaceae Metasequoia genus, is a critically endangered and protected species in China.
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